Detailed information of TA system TA163347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5808..6333
• Replicon: plasmid pRHBSTW-00636_3
• Accession: NZ_CP056374
• Organism: Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: HV253_RS27185
• Protein ID: WP_004197633.1
• Coordinates: 5808..6113 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HV253_RS27190
• Protein ID: WP_004197642.1
• Coordinates: 6115..6333 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV253_RS27155	1518..2144	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
HV253_RS27160	2264..2443	+	180	WP_006788217.1	hypothetical protein	-
HV253_RS27165	2883..3677	-	795	WP_004197635.1	site-specific integrase	-
HV253_RS27170	3875..4891	-	1017	WP_004197639.1	hypothetical protein	-
HV253_RS27175	4888..5211	-	324	WP_004197641.1	hypothetical protein	-
HV253_RS27180	5238..5639	-	402	WP_072199347.1	hypothetical protein	-
HV253_RS27185	5808..6113	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV253_RS27190	6115..6333	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV253_RS27195	6873..7826	+	954	WP_168380100.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HV253_RS27200	7896..8593	+	698	Protein_10	IS1 family transposase	-
HV253_RS27205	8644..9363	-	720	WP_040119806.1	GntR family transcriptional regulator	-
HV253_RS27210	9400..10068	-	669	WP_048293710.1	cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..171701	171701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T163347 WP_004197633.1 NZ_CP056374:c6113-5808 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163347 NZ_CP072539:3992060-3992437 [Escherichia coli O128ac:H12]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCATTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAACCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT163347 WP_004197642.1 NZ_CP056374:c6333-6115 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163347 NZ_CP072539:3991650-3992009 [Escherichia coli O128ac:H12]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5