Detailed information of TA system TA163345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 27933..28576
• Replicon: plasmid pRHBSTW-00636_2
• Accession: NZ_CP056373
• Organism: Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7D6UII7
• Locus tag: HV253_RS26225
• Protein ID: WP_032425563.1
• Coordinates: 28160..28576 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HV253_RS26220
• Protein ID: WP_001261276.1
• Coordinates: 27933..28163 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV253_RS26180	23473..23586	+	114	WP_014343462.1	hypothetical protein	-
HV253_RS26185	23715..23972	+	258	WP_009310077.1	hypothetical protein	-
HV253_RS26190	24030..24809	-	780	WP_023287113.1	site-specific integrase	-
HV253_RS26195	25007..26023	-	1017	WP_009309921.1	hypothetical protein	-
HV253_RS26200	26057..26392	-	336	WP_009309920.1	hypothetical protein	-
HV253_RS26205	26442..26753	-	312	WP_009309919.1	hypothetical protein	-
HV253_RS26210	26811..27116	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
HV253_RS26215	27118..27336	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
HV253_RS26220	27933..28163	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV253_RS26225	28160..28576	+	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV253_RS26230	28617..29351	-	735	Protein_32	restriction endonuclease	-
HV253_RS26235	29353..30321	+	969	WP_013815099.1	IS5-like element IS903B family transposase	-
HV253_RS26240	30379..30561	-	183	Protein_34	DUF3644 domain-containing protein	-
HV253_RS26245	31223..32500	+	1278	WP_009309915.1	HlyD family secretion protein	-
HV253_RS26250	32490..32894	+	405	WP_181486925.1	peptidase C39	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..193688	193688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.56 Da
• Isoelectric Point: 8.5403

>T163345 WP_032425563.1 NZ_CP056373:28160-28576 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T163345 NZ_CP072539:3691729-3691947 [Escherichia coli O128ac:H12]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT163345 WP_001261276.1 NZ_CP056373:27933-28163 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT163345 NZ_CP072539:3691329-3691703 [Escherichia coli O128ac:H12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7D6UII7

Antitoxin

• AlphaFold DB: A0A387K023