Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26811..27336 Replicon plasmid pRHBSTW-00636_2
Accession NZ_CP056373
Organism Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag HV253_RS26210 Protein ID WP_009309918.1
Coordinates 26811..27116 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV253_RS26215 Protein ID WP_006788213.1
Coordinates 27118..27336 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV253_RS26175 21973..22941 + 969 WP_000654811.1 IS5 family transposase -
HV253_RS26180 23473..23586 + 114 WP_014343462.1 hypothetical protein -
HV253_RS26185 23715..23972 + 258 WP_009310077.1 hypothetical protein -
HV253_RS26190 24030..24809 - 780 WP_023287113.1 site-specific integrase -
HV253_RS26195 25007..26023 - 1017 WP_009309921.1 hypothetical protein -
HV253_RS26200 26057..26392 - 336 WP_009309920.1 hypothetical protein -
HV253_RS26205 26442..26753 - 312 WP_009309919.1 hypothetical protein -
HV253_RS26210 26811..27116 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
HV253_RS26215 27118..27336 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV253_RS26220 27933..28163 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV253_RS26225 28160..28576 + 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin -
HV253_RS26230 28617..29351 - 735 Protein_32 restriction endonuclease -
HV253_RS26235 29353..30321 + 969 WP_013815099.1 IS5-like element IS903B family transposase -
HV253_RS26240 30379..30561 - 183 Protein_34 DUF3644 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..193688 193688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T163344 WP_009309918.1 NZ_CP056373:c27116-26811 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T163344 NZ_CP072539:2911141-2911248 [Escherichia coli O128ac:H12]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT163344 WP_006788213.1 NZ_CP056373:c27336-27118 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT163344 NZ_CP072539:c2911091-2911028 [Escherichia coli O128ac:H12]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References