Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5212350..5212975 Replicon chromosome
Accession NZ_CP056372
Organism Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV253_RS25615 Protein ID WP_002882817.1
Coordinates 5212350..5212733 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV253_RS25620 Protein ID WP_004150355.1
Coordinates 5212733..5212975 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV253_RS25600 5209716..5210618 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV253_RS25605 5210615..5211250 + 636 WP_064147423.1 formate dehydrogenase cytochrome b556 subunit -
HV253_RS25610 5211247..5212176 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV253_RS25615 5212350..5212733 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV253_RS25620 5212733..5212975 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV253_RS25625 5213180..5214097 + 918 WP_064147424.1 alpha/beta hydrolase -
HV253_RS25630 5214111..5215052 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV253_RS25635 5215097..5215534 - 438 WP_064147425.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV253_RS25640 5215531..5216391 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV253_RS25645 5216385..5216984 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163343 WP_002882817.1 NZ_CP056372:c5212733-5212350 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163343 NZ_CP072539:2910606-2910713 [Escherichia coli O128ac:H12]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163343 WP_004150355.1 NZ_CP056372:c5212975-5212733 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163343 NZ_CP072539:c2910559-2910493 [Escherichia coli O128ac:H12]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References