Detailed information of TA system TA163340

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4264190..4264944
• Replicon: chromosome
• Accession: NZ_CP056372
• Organism: Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A377E3D6
• Locus tag: HV253_RS21120
• Protein ID: WP_001194695.1
• Coordinates: 4264567..4264944 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV253_RS21115
• Protein ID: WP_165688905.1
• Coordinates: 4264190..4264516 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV253_RS21070	4259396..4259848	+	453	WP_001020418.1	hypothetical protein	-
HV253_RS21075	4259845..4260297	+	453	WP_000734138.1	hypothetical protein	-
HV253_RS21080	4260360..4260902	+	543	WP_001104018.1	DUF4339 domain-containing protein	-
HV253_RS21085	4260962..4261414	+	453	WP_001061894.1	hypothetical protein	-
HV253_RS21090	4261491..4261724	+	234	WP_001114682.1	DUF905 domain-containing protein	-
HV253_RS21095	4261844..4262662	+	819	WP_001234406.1	DUF945 domain-containing protein	-
HV253_RS21100	4262930..4263400	+	471	WP_000131762.1	antirestriction protein	-
HV253_RS21105	4263412..4263891	+	480	WP_064159939.1	DNA repair protein RadC	-
HV253_RS21110	4263912..4264133	+	222	WP_000691981.1	DUF987 domain-containing protein	-
HV253_RS21115	4264190..4264516	+	327	WP_165688905.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV253_RS21120	4264567..4264944	+	378	WP_001194695.1	TA system toxin CbtA family protein	Toxin
HV253_RS21125	4264941..4265432	+	492	WP_000777682.1	hypothetical protein	-
HV253_RS21130	4265464..4265667	+	204	WP_000413747.1	DUF957 domain-containing protein	-
HV253_RS21135	4265748..4266592	+	845	Protein_4151	DUF4942 domain-containing protein	-
HV253_RS21145	4266935..4267675	-	741	WP_004145831.1	DNA polymerase III subunit epsilon	-
HV253_RS21150	4267731..4268198	+	468	WP_002889686.1	ribonuclease HI	-
HV253_RS21155	4268195..4268917	-	723	WP_002889685.1	class I SAM-dependent methyltransferase	-
HV253_RS21160	4268950..4269705	+	756	WP_004152035.1	hydroxyacylglutathione hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14180.97 Da
• Isoelectric Point: 7.4209

>T163340 WP_001194695.1 NZ_CP056372:4264567-4264944 [Klebsiella pneumoniae]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Nucleotide

• Length: 378 bp

>T163340 NZ_CP072539:2504985-2505272 [Escherichia coli O128ac:H12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11889.62 Da
• Isoelectric Point: 6.9453

>AT163340 WP_165688905.1 NZ_CP056372:4264190-4264516 [Klebsiella pneumoniae]
ITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELTPRHQHCVTLYA
KGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT163340 NZ_CP072539:2504746-2504985 [Escherichia coli O128ac:H12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A377E3D6