Detailed information of TA system TA163329

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 789200..789857
• Replicon: chromosome
• Accession: NZ_CP056372
• Organism: Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W8UCT0
• Locus tag: HV253_RS03920
• Protein ID: WP_002916310.1
• Coordinates: 789447..789857 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HV253_RS03915
• Protein ID: WP_181486880.1
• Coordinates: 789200..789466 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV253_RS03890	784408..785841	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
HV253_RS03895	785960..786688	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
HV253_RS03900	786738..787049	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
HV253_RS03905	787213..787872	+	660	WP_064159882.1	hemolysin III family protein	-
HV253_RS03910	787971..788954	-	984	WP_023286864.1	tRNA-modifying protein YgfZ	-
HV253_RS03915	789200..789466	+	267	WP_181486880.1	FAD assembly factor SdhE	Antitoxin
HV253_RS03920	789447..789857	+	411	WP_002916310.1	protein YgfX	Toxin
HV253_RS03925	789864..790385	-	522	WP_002916308.1	flavodoxin FldB	-
HV253_RS03930	790486..791382	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
HV253_RS03935	791405..792118	+	714	WP_004174456.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV253_RS03940	792124..793857	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16049.85 Da
• Isoelectric Point: 11.4778

>T163329 WP_002916310.1 NZ_CP056372:789447-789857 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T163329 NZ_CP072539:445424-445699 [Escherichia coli O128ac:H12]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10452.92 Da
• Isoelectric Point: 4.8053

>AT163329 WP_181486880.1 NZ_CP056372:789200-789466 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQQMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT163329 NZ_CP072539:445696-446055 [Escherichia coli O128ac:H12]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GSW7