Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4868724..4869340 Replicon chromosome
Accession NZ_CP056365
Organism Citrobacter freundii strain RHBSTW-00658

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HV257_RS23210 Protein ID WP_003028682.1
Coordinates 4868724..4869098 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HV257_RS23215 Protein ID WP_043018956.1
Coordinates 4869098..4869340 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV257_RS23195 4866227..4867129 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV257_RS23200 4867126..4867761 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV257_RS23205 4867758..4868687 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV257_RS23210 4868724..4869098 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HV257_RS23215 4869098..4869340 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HV257_RS23220 4869546..4870454 + 909 WP_044701816.1 alpha/beta hydrolase -
HV257_RS23225 4870605..4871546 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV257_RS23230 4871591..4872028 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV257_RS23235 4872025..4872897 - 873 WP_003028669.1 virulence factor BrkB family protein -
HV257_RS23240 4872891..4873490 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T163325 WP_003028682.1 NZ_CP056365:c4869098-4868724 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T163325 NZ_CP072528:69120-69440 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT163325 WP_043018956.1 NZ_CP056365:c4869340-4869098 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT163325 NZ_CP072528:69433-69705 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References