163324

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 4685144..4685810 Replicon chromosome
Accession NZ_CP056365
Organism Citrobacter freundii strain RHBSTW-00658

Toxin (Protein)


Gene name tad Uniprot ID A0A0D7LIM5
Locus tag HV257_RS22315 Protein ID WP_044702260.1
Coordinates 4685451..4685810 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0D7LIR0
Locus tag HV257_RS22310 Protein ID WP_003844682.1
Coordinates 4685144..4685461 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV257_RS22280 4680811..4681635 + 825 WP_003031641.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HV257_RS22285 4681704..4682927 + 1224 WP_044702265.1 L-sorbose 1-phosphate reductase -
HV257_RS22290 4682929..4683741 + 813 WP_044702264.1 shikimate 5-dehydrogenase -
HV257_RS22295 4683793..4684149 - 357 WP_032948294.1 hypothetical protein -
HV257_RS22300 4684291..4684452 + 162 WP_003841416.1 phage protein -
HV257_RS22305 4684856..4685134 + 279 WP_044702262.1 putative addiction module antidote protein -
HV257_RS22310 4685144..4685461 - 318 WP_003844682.1 helix-turn-helix domain-containing protein Antitoxin
HV257_RS22315 4685451..4685810 - 360 WP_044702260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV257_RS22320 4686024..4686713 + 690 WP_003841421.1 dipeptidase PepE -
HV257_RS22325 4686779..4688410 - 1632 WP_032938230.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-90)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13437.49 Da        Isoelectric Point: 10.2555

>T163324 WP_044702260.1 NZ_CP056365:c4685810-4685451 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Download         Length: 360 bp

>T163324 NZ_CP072528:52155-52442 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11466.35 Da        Isoelectric Point: 9.0005

>AT163324 WP_003844682.1 NZ_CP056365:c4685461-4685144 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Download         Length: 318 bp

>AT163324 NZ_CP072528:52429-52743 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LIM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LIR0

References