Detailed information of TA system TA163323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4429012..4429528
• Replicon: chromosome
• Accession: NZ_CP056365
• Organism: Citrobacter freundii strain RHBSTW-00658

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV257_RS21095
• Protein ID: WP_044699148.1
• Coordinates: 4429012..4429296 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: HV257_RS21100
• Protein ID: WP_003839576.1
• Coordinates: 4429286..4429528 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV257_RS21080	4425337..4425921	+	585	WP_003839584.1	fructose PTS transporter subunit IIA	-
HV257_RS21085	4426299..4428437	+	2139	WP_003025782.1	anaerobic ribonucleoside-triphosphate reductase	-
HV257_RS21090	4428544..4429008	+	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HV257_RS21095	4429012..4429296	-	285	WP_044699148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV257_RS21100	4429286..4429528	-	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HV257_RS21105	4429606..4431519	-	1914	WP_003839574.1	BglG family transcription antiterminator	-
HV257_RS21110	4431541..4432281	-	741	WP_044699149.1	KDGP aldolase family protein	-
HV257_RS21115	4432278..4433396	-	1119	WP_181548299.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HV257_RS21120	4433380..4434513	-	1134	WP_016149471.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10865.69 Da
• Isoelectric Point: 10.1988

>T163323 WP_044699148.1 NZ_CP056365:c4429296-4429012 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
RQHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T163323 NZ_CP072528:c51207-50905 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT163323 WP_003839576.1 NZ_CP056365:c4429528-4429286 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT163323 NZ_CP072528:c50904-50623 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10