163321

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3980288..3980967 Replicon chromosome
Accession NZ_CP056365
Organism Citrobacter freundii strain RHBSTW-00658

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV257_RS19065 Protein ID WP_003031349.1
Coordinates 3980626..3980967 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV257_RS19060 Protein ID WP_003031347.1
Coordinates 3980288..3980605 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV257_RS19035 3976381..3978378 - 1998 WP_016245729.1 choline BCCT transporter BetT -
HV257_RS19040 3978882..3979073 + 192 Protein_3732 DUF905 family protein -
HV257_RS19045 3979087..3979548 + 462 WP_003031344.1 antirestriction protein -
HV257_RS19050 3979564..3980040 + 477 WP_003031345.1 RadC family protein -
HV257_RS19055 3980049..3980270 + 222 WP_003031346.1 DUF987 domain-containing protein -
HV257_RS19060 3980288..3980605 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV257_RS19065 3980626..3980967 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV257_RS19075 3981597..3982061 - 465 WP_044702482.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV257_RS19080 3982253..3983701 - 1449 WP_044702495.1 EAL domain-containing protein -
HV257_RS19085 3984043..3984516 - 474 WP_044702484.1 hypothetical protein -
HV257_RS19090 3984491..3985222 - 732 WP_044702485.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3965915..3992863 26948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T163321 WP_003031349.1 NZ_CP056365:3980626-3980967 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163321 NZ_CP072526:c3270100-3269711 [Acinetobacter baumannii]
TTGATCAACCATTCGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCCTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCGGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCCATTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT163321 WP_003031347.1 NZ_CP056365:3980288-3980605 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT163321 NZ_CP072526:c3270364-3270107 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References