Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3744634..3745254 Replicon chromosome
Accession NZ_CP056365
Organism Citrobacter freundii strain RHBSTW-00658

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV257_RS17960 Protein ID WP_002892050.1
Coordinates 3745036..3745254 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV257_RS17955 Protein ID WP_003021733.1
Coordinates 3744634..3745008 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV257_RS17945 3739781..3740974 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV257_RS17950 3740997..3744146 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV257_RS17955 3744634..3745008 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV257_RS17960 3745036..3745254 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV257_RS17965 3745561..3746112 + 552 WP_044699372.1 maltose O-acetyltransferase -
HV257_RS17970 3746229..3746699 + 471 WP_003021724.1 YlaC family protein -
HV257_RS17975 3746778..3746918 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV257_RS17980 3746920..3747180 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HV257_RS17985 3747369..3748922 + 1554 WP_044699371.1 EAL domain-containing protein -
HV257_RS17990 3748974..3749327 - 354 WP_003831033.1 DUF1428 family protein -
HV257_RS17995 3749392..3750021 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163320 WP_002892050.1 NZ_CP056365:3745036-3745254 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163320 NZ_CP072525:2521863-2522042 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATACATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163320 WP_003021733.1 NZ_CP056365:3744634-3745008 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163320 NZ_CP072525:c2522188-2521966 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References