Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5190025..5190650 Replicon chromosome
Accession NZ_CP056329
Organism Klebsiella pneumoniae strain RHBSTW-00807

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV282_RS25430 Protein ID WP_002882817.1
Coordinates 5190025..5190408 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV282_RS25435 Protein ID WP_004150355.1
Coordinates 5190408..5190650 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV282_RS25415 5187391..5188293 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV282_RS25420 5188290..5188925 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV282_RS25425 5188922..5189851 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV282_RS25430 5190025..5190408 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV282_RS25435 5190408..5190650 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV282_RS25440 5190855..5191772 + 918 WP_012737090.1 alpha/beta hydrolase -
HV282_RS25445 5191787..5192728 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV282_RS25450 5192773..5193210 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV282_RS25455 5193207..5194067 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV282_RS25460 5194061..5194660 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163307 WP_002882817.1 NZ_CP056329:c5190408-5190025 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163307 NZ_CP072518:c25762-25280 [Klebsiella quasipneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163307 WP_004150355.1 NZ_CP056329:c5190650-5190408 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163307 NZ_CP072518:c26016-25750 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References