Detailed information of TA system TA163302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2614955..2616543
• Replicon: chromosome
• Accession: NZ_CP056329
• Organism: Klebsiella pneumoniae strain RHBSTW-00807

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HV282_RS13000
• Protein ID: WP_135717380.1
• Coordinates: 2614955..2616277 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: HV282_RS13005
• Protein ID: WP_002904790.1
• Coordinates: 2616277..2616543 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV282_RS12985	2610011..2612512	+	2502	WP_038807929.1	fimbrial biogenesis outer membrane usher protein	-
HV282_RS12990	2612500..2613504	+	1005	WP_038807930.1	type 1 fimbrial protein	-
HV282_RS12995	2613646..2614938	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HV282_RS13000	2614955..2616277	-	1323	WP_135717380.1	type II toxin-antitoxin system HipA family toxin	Toxin
HV282_RS13005	2616277..2616543	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HV282_RS13010	2616809..2617135	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
HV282_RS13015	2617132..2617632	-	501	WP_032411470.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HV282_RS13020	2617610..2618989	-	1380	WP_032411468.1	purine permease	-
HV282_RS13025	2619335..2620489	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HV282_RS13030	2620470..2621393	-	924	WP_032411466.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49187.52 Da
• Isoelectric Point: 7.5062

>T163302 WP_135717380.1 NZ_CP056329:c2616277-2614955 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITCQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T163302 NZ_CP072515:2492303-2493625 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGGCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGAAGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGAATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACTCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCAAACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT163302 WP_002904790.1 NZ_CP056329:c2616543-2616277 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT163302 NZ_CP072515:2492037-2492303 [Klebsiella quasipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8