Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3722707..3723327 Replicon chromosome
Accession NZ_CP056314
Organism Citrobacter freundii strain RHBSTW-00830

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV290_RS17865 Protein ID WP_002892050.1
Coordinates 3723109..3723327 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV290_RS17860 Protein ID WP_003021733.1
Coordinates 3722707..3723081 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV290_RS17850 3717853..3719046 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV290_RS17855 3719069..3722218 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV290_RS17860 3722707..3723081 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV290_RS17865 3723109..3723327 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV290_RS17870 3723634..3724185 + 552 WP_003835924.1 maltose O-acetyltransferase -
HV290_RS17875 3724302..3724772 + 471 WP_003021724.1 YlaC family protein -
HV290_RS17880 3724851..3724991 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV290_RS17885 3724993..3725253 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HV290_RS17890 3725442..3726995 + 1554 WP_044699371.1 EAL domain-containing protein -
HV290_RS17895 3727047..3727400 - 354 WP_003831033.1 DUF1428 family protein -
HV290_RS17900 3727465..3728094 - 630 WP_003021707.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163285 WP_002892050.1 NZ_CP056314:3723109-3723327 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163285 NZ_CP072496:328901-329269 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163285 WP_003021733.1 NZ_CP056314:3722707-3723081 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163285 NZ_CP072496:328683-328904 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTAACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References