Detailed information of TA system TA163283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2549157..2549796
• Replicon: chromosome
• Accession: NZ_CP056314
• Organism: Citrobacter freundii strain RHBSTW-00830

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV290_RS12175
• Protein ID: WP_003020221.1
• Coordinates: 2549620..2549796 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV290_RS12170
• Protein ID: WP_123924900.1
• Coordinates: 2549157..2549573 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV290_RS12150	2544203..2545147	-	945	WP_003843727.1	ABC transporter permease	-
HV290_RS12155	2545148..2546164	-	1017	WP_058842662.1	ABC transporter ATP-binding protein	-
HV290_RS12160	2546181..2547326	-	1146	WP_061548295.1	ABC transporter substrate-binding protein	-
HV290_RS12165	2547655..2549064	-	1410	WP_003843728.1	PLP-dependent aminotransferase family protein	-
HV290_RS12170	2549157..2549573	-	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV290_RS12175	2549620..2549796	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV290_RS12180	2550060..2551463	+	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
HV290_RS12185	2551463..2552473	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
HV290_RS12190	2552473..2552598	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV290_RS12195	2552788..2553018	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T163283 WP_003020221.1 NZ_CP056314:c2549796-2549620 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T163283 NZ_CP072495:c67682-67200 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT163283 WP_123924900.1 NZ_CP056314:c2549573-2549157 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT163283 NZ_CP072495:c67936-67670 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36