Detailed information of TA system TA163272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 250841..251472
• Replicon: chromosome
• Accession: NZ_CP056314
• Organism: Citrobacter freundii strain RHBSTW-00830

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HV290_RS01155
• Protein ID: WP_046671263.1
• Coordinates: 250841..251116 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1MMS0
• Locus tag: HV290_RS01160
• Protein ID: WP_003837808.1
• Coordinates: 251113..251472 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV290_RS01145	246892..249639	+	2748	WP_049025788.1	ribosome-associated ATPase/putative transporter RbbA	-
HV290_RS01150	249639..250763	+	1125	WP_086529765.1	ABC transporter permease	-
HV290_RS01155	250841..251116	+	276	WP_046671263.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV290_RS01160	251113..251472	+	360	WP_003837808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV290_RS01165	251585..251986	-	402	WP_003023387.1	nickel-responsive transcriptional regulator NikR	-
HV290_RS01170	252036..252614	-	579	WP_071697729.1	4'-phosphopantetheinyl transferase AcpT	-
HV290_RS01175	252677..253726	-	1050	WP_003023393.1	AI-2E family transporter	-
HV290_RS01180	253860..255077	+	1218	WP_016151126.1	MFS transporter	-
HV290_RS01185	255081..255638	-	558	WP_003837816.1	DcrB family lipoprotein	-
HV290_RS01190	255711..256376	-	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10238.90 Da
• Isoelectric Point: 10.7844

>T163272 WP_046671263.1 NZ_CP056314:250841-251116 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIRWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T163272 NZ_CP072494:324245-324613 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13198.98 Da
• Isoelectric Point: 4.5462

>AT163272 WP_003837808.1 NZ_CP056314:251113-251472 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT163272 NZ_CP072494:324027-324248 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MMS0