Detailed information of TA system TA163271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 66485..67221
• Replicon: plasmid pRHBSTW-00832_2
• Accession: NZ_CP056308
• Organism: Klebsiella pneumoniae strain RHBSTW-00832

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV292_RS27360
• Protein ID: WP_181247937.1
• Coordinates: 66739..67221 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HV292_RS27355
• Protein ID: WP_003026799.1
• Coordinates: 66485..66751 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV292_RS27315	61848..63386	+	1539	WP_004196907.1	IS66-like element ISEc22 family transposase	-
HV292_RS27320	63676..63939	+	264	WP_009310051.1	hypothetical protein	-
HV292_RS27325	63936..64502	+	567	WP_009310052.1	hypothetical protein	-
HV292_RS27330	64533..65027	+	495	WP_009310053.1	hypothetical protein	-
HV292_RS27335	65071..65439	+	369	WP_009310054.1	hypothetical protein	-
HV292_RS27340	65470..65673	+	204	WP_004098931.1	hemolysin expression modulator Hha	-
HV292_RS27345	65722..65979	+	258	WP_004098928.1	hypothetical protein	-
HV292_RS27350	66055..66309	+	255	WP_004118702.1	hypothetical protein	-
HV292_RS27355	66485..66751	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HV292_RS27360	66739..67221	+	483	WP_181247937.1	GNAT family N-acetyltransferase	Toxin
HV292_RS27365	67381..68913	-	1533	WP_009310015.1	IS3-like element ISKpn38 family transposase	-
HV292_RS27370	69025..70428	+	1404	WP_181247936.1	ISNCY-like element ISKpn21 family transposase	-
HV292_RS27375	70457..71089	-	633	WP_001567369.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..232875	232875
	-	inside	IScluster/Tn	-	-	61051..84734	23683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17311.89 Da
• Isoelectric Point: 9.5822

>T163271 WP_181247937.1 NZ_CP056308:66739-67221 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGGGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T163271 NZ_CP072493:c156505-156062 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCACGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT163271 WP_003026799.1 NZ_CP056308:66485-66751 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163271 NZ_CP072493:c156732-156502 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66