Detailed information of TA system TA163269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 23004..23647
• Replicon: plasmid pRHBSTW-00832_2
• Accession: NZ_CP056308
• Organism: Klebsiella pneumoniae strain RHBSTW-00832

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV292_RS27115
• Protein ID: WP_009309917.1
• Coordinates: 23231..23647 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HV292_RS27110
• Protein ID: WP_001261276.1
• Coordinates: 23004..23234 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV292_RS27070	18544..18657	+	114	WP_014343462.1	hypothetical protein	-
HV292_RS27075	18786..19043	+	258	WP_009310077.1	hypothetical protein	-
HV292_RS27080	19101..19880	-	780	WP_023287113.1	site-specific integrase	-
HV292_RS27085	20078..21094	-	1017	WP_009309921.1	hypothetical protein	-
HV292_RS27090	21128..21463	-	336	WP_009309920.1	hypothetical protein	-
HV292_RS27095	21513..21824	-	312	WP_009309919.1	hypothetical protein	-
HV292_RS27100	21882..22187	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
HV292_RS27105	22189..22407	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
HV292_RS27110	23004..23234	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV292_RS27115	23231..23647	+	417	WP_009309917.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV292_RS27120	23688..24566	-	879	WP_009309916.1	restriction endonuclease	-
HV292_RS27125	25228..26505	+	1278	WP_009309915.1	HlyD family secretion protein	-
HV292_RS27130	26495..28603	+	2109	WP_032427226.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..232875	232875
	-	flank	IS/Tn	-	-	17461..18441	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T163269 WP_009309917.1 NZ_CP056308:23231-23647 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKGSPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T163269 NZ_CP072493:c58150-57839 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT163269 WP_001261276.1 NZ_CP056308:23004-23234 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT163269 NZ_CP072493:c57842-57423 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023