Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21882..22407 Replicon plasmid pRHBSTW-00832_2
Accession NZ_CP056308
Organism Klebsiella pneumoniae strain RHBSTW-00832

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag HV292_RS27100 Protein ID WP_009309918.1
Coordinates 21882..22187 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV292_RS27105 Protein ID WP_006788213.1
Coordinates 22189..22407 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV292_RS27065 17461..18441 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
HV292_RS27070 18544..18657 + 114 WP_014343462.1 hypothetical protein -
HV292_RS27075 18786..19043 + 258 WP_009310077.1 hypothetical protein -
HV292_RS27080 19101..19880 - 780 WP_023287113.1 site-specific integrase -
HV292_RS27085 20078..21094 - 1017 WP_009309921.1 hypothetical protein -
HV292_RS27090 21128..21463 - 336 WP_009309920.1 hypothetical protein -
HV292_RS27095 21513..21824 - 312 WP_009309919.1 hypothetical protein -
HV292_RS27100 21882..22187 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
HV292_RS27105 22189..22407 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV292_RS27110 23004..23234 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV292_RS27115 23231..23647 + 417 WP_009309917.1 type II toxin-antitoxin system VapC family toxin -
HV292_RS27120 23688..24566 - 879 WP_009309916.1 restriction endonuclease -
HV292_RS27125 25228..26505 + 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..232875 232875
- flank IS/Tn - - 17461..18441 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T163268 WP_009309918.1 NZ_CP056308:c22187-21882 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T163268 NZ_CP072492:c5319951-5319568 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT163268 WP_006788213.1 NZ_CP056308:c22407-22189 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT163268 NZ_CP072492:c5320193-5319951 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References