Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5381392..5382017 Replicon chromosome
Accession NZ_CP056307
Organism Klebsiella pneumoniae strain RHBSTW-00832

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV292_RS26605 Protein ID WP_002882817.1
Coordinates 5381392..5381775 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV292_RS26610 Protein ID WP_004150355.1
Coordinates 5381775..5382017 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV292_RS26590 5378758..5379660 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV292_RS26595 5379657..5380292 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV292_RS26600 5380289..5381218 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV292_RS26605 5381392..5381775 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV292_RS26610 5381775..5382017 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV292_RS26615 5382222..5383139 + 918 WP_009309725.1 alpha/beta hydrolase -
HV292_RS26620 5383153..5384094 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV292_RS26625 5384139..5384576 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV292_RS26630 5384573..5385433 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV292_RS26635 5385427..5386026 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163267 WP_002882817.1 NZ_CP056307:c5381775-5381392 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163267 NZ_CP072492:c4849002-4848718 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163267 WP_004150355.1 NZ_CP056307:c5382017-5381775 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163267 NZ_CP072492:c4849234-4848992 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References