Detailed information of TA system TA163249

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 691857..692560
• Replicon: chromosome
• Accession: NZ_CP056307
• Organism: Klebsiella pneumoniae strain RHBSTW-00832

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV292_RS03450
• Protein ID: WP_009309675.1
• Coordinates: 692219..692560 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV292_RS03445
• Protein ID: WP_009309674.1
• Coordinates: 691857..692198 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV292_RS03420	687373..688535	+	1163	WP_087728260.1	IS3 family transposase	-
HV292_RS03425	688936..689799	+	864	WP_009309669.1	hypothetical protein	-
HV292_RS03430	689997..690818	+	822	WP_009309670.1	DUF945 domain-containing protein	-
HV292_RS03435	690849..691291	+	443	Protein_675	antirestriction protein	-
HV292_RS03440	691304..691846	+	543	WP_009309673.1	DNA repair protein RadC	-
HV292_RS03445	691857..692198	+	342	WP_009309674.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV292_RS03450	692219..692560	+	342	WP_009309675.1	TA system toxin CbtA family protein	Toxin
HV292_RS03455	692853..693890	-	1038	WP_181247888.1	tyrosine-type recombinase/integrase	-
HV292_RS03460	693897..694481	-	585	WP_057222572.1	phage repressor protein CI	-
HV292_RS03465	694601..694822	+	222	WP_004195891.1	hypothetical protein	-
HV292_RS03470	694853..695362	+	510	WP_181247889.1	phage regulatory CII family protein	-
HV292_RS03475	695370..695570	+	201	WP_181247890.1	DUF2724 domain-containing protein	-
HV292_RS03480	695651..695938	+	288	WP_181247931.1	hypothetical protein	-
HV292_RS03485	696004..696228	+	225	WP_143911932.1	DUF2732 family protein	-
HV292_RS03490	696228..696455	+	228	WP_023317535.1	TraR/DksA family transcriptional regulator	-
HV292_RS03495	696452..697033	+	582	WP_181247891.1	3'-5' exonuclease	-
HV292_RS03500	697030..697302	+	273	WP_135712807.1	DUF5405 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	684146..732517	48371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12822.76 Da
• Isoelectric Point: 9.6542

>T163249 WP_009309675.1 NZ_CP056307:692219-692560 [Klebsiella pneumoniae]
MKTLPATISRAAKPCLSPVAAWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAEITLADAVNFLVDKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLRRSRNNAVS

Nucleotide

• Length: 342 bp

>T163249 NZ_CP072467:c1255056-1254637 [Spiractinospora alimapuensis]
ATGATTGTCCTGGACACGAACGTCATCTCCGAGATCTTCCGACCGAACCCCGACGAGCGAGTCGTCACGTGGCTGGGGTC
CCTCACCGGGGAGGTTGCCGTCACCGCCGTCACGCTGGCTGAGCTTCGGGCTGGTGTTGGCCGTCTCCCAGAGGGTAAGC
GCAGATCGGCGCTTCAGATGCGGATCGAGGCTGCGCTTCAGCCCTACCGGGAAACCCGAGCGATACTGGTCTTCGACGAC
GACGCGGCTGGACACTACGCGGAGATCGTCGTGGCGCGCGAAAGGGCGGGCATCCCGATCAGCATGGCCGACGCGCAGAT
CGCCGCGATGTGCCGGAGCAACAGCGCCACCTGCGCTACGCGGAACACGAAAGACTTCACCGAGACTGGGATCGAGCTGA
TCAACCCGTGGGACGAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12735.41 Da
• Isoelectric Point: 6.3157

>AT163249 WP_009309674.1 NZ_CP056307:691857-692198 [Klebsiella pneumoniae]
MKSTESENDLFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGAFSNAESLKLDEVFPRFISQMESMLTIGEMNPHH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT163249 NZ_CP072467:c1255286-1255053 [Spiractinospora alimapuensis]
ATGGCGTCCATAGTCGTTCGAGGGCTCGACGAGTCAGTGAAAAAGCAGATCGCGATCCAGGCGAAGGAGCACGGGCACTC
CATGGAAGCCGAGGTTCGTGAGATCCTCACGAGGGCGGCTGGACGGCCGAACATTGGCATTGCCCTCATGAGGGCCGTGA
TGGACGTTGGCGGAGTCGACGATCTCCCGGTTCCCGTGCGCACCGATGCCGCGCGGGTGGTGGACTTCGGATGA