Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5219329..5219954 Replicon chromosome
Accession NZ_CP056301
Organism Klebsiella pneumoniae strain RHBSTW-00843

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV294_RS25145 Protein ID WP_002882817.1
Coordinates 5219329..5219712 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV294_RS25150 Protein ID WP_004150355.1
Coordinates 5219712..5219954 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV294_RS25130 5216695..5217597 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV294_RS25135 5217594..5218229 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV294_RS25140 5218226..5219155 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV294_RS25145 5219329..5219712 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV294_RS25150 5219712..5219954 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV294_RS25155 5220159..5221076 + 918 WP_021466676.1 alpha/beta hydrolase -
HV294_RS25160 5221090..5222031 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV294_RS25165 5222076..5222513 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV294_RS25170 5222510..5223370 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV294_RS25175 5223364..5223963 - 600 WP_130958667.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163245 WP_002882817.1 NZ_CP056301:c5219712-5219329 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163245 NZ_CP072466:c10487-10173 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163245 WP_004150355.1 NZ_CP056301:c5219954-5219712 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163245 NZ_CP072466:c10753-10475 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References