Detailed information of TA system TA163242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4596243..4597053
• Replicon: chromosome
• Accession: NZ_CP056301
• Organism: Klebsiella pneumoniae strain RHBSTW-00843

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV294_RS22255
• Protein ID: WP_048299784.1
• Coordinates: 4596243..4596776 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HV294_RS22260
• Protein ID: WP_002887278.1
• Coordinates: 4596787..4597053 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV294_RS22250	4595074..4596195	+	1122	WP_004214138.1	cupin domain-containing protein	-
HV294_RS22255	4596243..4596776	-	534	WP_048299784.1	GNAT family N-acetyltransferase	Toxin
HV294_RS22260	4596787..4597053	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HV294_RS22265	4597156..4598589	-	1434	WP_040210879.1	Cu(+)/Ag(+) sensor histidine kinase	-
HV294_RS22270	4598579..4599262	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HV294_RS22275	4599434..4600819	+	1386	WP_040089708.1	efflux transporter outer membrane subunit	-
HV294_RS22280	4600837..4601181	+	345	WP_023287080.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19826.65 Da
• Isoelectric Point: 5.2614

>T163242 WP_048299784.1 NZ_CP056301:c4596776-4596243 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGYDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T163242 NZ_CP072463:c5543832-5543449 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT163242 WP_002887278.1 NZ_CP056301:c4597053-4596787 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT163242 NZ_CP072463:c5544074-5543832 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1