Detailed information of TA system TA163231

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 804497..805154
• Replicon: chromosome
• Accession: NZ_CP056301
• Organism: Klebsiella pneumoniae strain RHBSTW-00843

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W8UCT0
• Locus tag: HV294_RS03955
• Protein ID: WP_002916310.1
• Coordinates: 804744..805154 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HV294_RS03950
• Protein ID: WP_181467330.1
• Coordinates: 804497..804763 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV294_RS03925	799653..801086	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
HV294_RS03930	801205..801933	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
HV294_RS03935	801983..802294	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
HV294_RS03940	802458..803117	+	660	WP_002916317.1	hemolysin III family protein	-
HV294_RS03945	803268..804251	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
HV294_RS03950	804497..804763	+	267	WP_181467330.1	FAD assembly factor SdhE	Antitoxin
HV294_RS03955	804744..805154	+	411	WP_002916310.1	protein YgfX	Toxin
HV294_RS03960	805161..805682	-	522	WP_004144730.1	flavodoxin FldB	-
HV294_RS03965	805783..806679	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
HV294_RS03970	806702..807415	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV294_RS03975	807421..809154	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16049.85 Da
• Isoelectric Point: 11.4778

>T163231 WP_002916310.1 NZ_CP056301:804744-805154 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T163231 NZ_CP072463:355289-355636 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10477.96 Da
• Isoelectric Point: 5.7035

>AT163231 WP_181467330.1 NZ_CP056301:804497-804763 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIKPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT163231 NZ_CP072463:355636-355935 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GSW7