Detailed information of TA system TA163229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 73930..74452
• Replicon: plasmid pRHBSTW-00852_2
• Accession: NZ_CP056298
• Organism: Klebsiella pneumoniae strain RHBSTW-00852

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: HV298_RS25400
• Protein ID: WP_004187019.1
• Coordinates: 74168..74452 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HV298_RS25395
• Protein ID: WP_004187025.1
• Coordinates: 73930..74178 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV298_RS25365	71297..71866	-	570	WP_004152117.1	small heat shock protein sHSP20	-
HV298_RS25370	71901..72182	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
HV298_RS25375	72426..72689	-	264	WP_004118208.1	hypothetical protein	-
HV298_RS25380	72704..72967	+	264	WP_004187027.1	transposase	-
HV298_RS25385	72912..73028	+	117	Protein_70	transposase domain-containing protein	-
HV298_RS25390	73216..73640	+	425	Protein_71	IS1 family transposase	-
HV298_RS25395	73930..74178	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HV298_RS25400	74168..74452	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV298_RS25405	74468..75823	-	1356	WP_004187017.1	ISNCY family transposase	-
HV298_RS25410	75905..76204	-	300	Protein_75	conjugal transfer protein TraH	-
HV298_RS25415	76202..76685	-	484	Protein_76	hypothetical protein	-
HV298_RS25420	76702..77226	-	525	Protein_77	hypothetical protein	-
HV298_RS25425	78769..79233	+	465	WP_004187010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131105	131105
	-	flank	IS/Tn	-	-	73410..73676	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T163229 WP_004187019.1 NZ_CP056298:74168-74452 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T163229 NZ_CP072461:c179828-179412 [Klebsiella pneumoniae]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT163229 WP_004187025.1 NZ_CP056298:73930-74178 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT163229 NZ_CP072461:c180055-179825 [Klebsiella pneumoniae]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4