Detailed information of TA system TA163228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 60299..61035
• Replicon: plasmid pRHBSTW-00852_2
• Accession: NZ_CP056298
• Organism: Klebsiella pneumoniae strain RHBSTW-00852

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: HV298_RS25310
• Protein ID: WP_004187044.1
• Coordinates: 60299..60781 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HV298_RS25315
• Protein ID: WP_003026799.1
• Coordinates: 60769..61035 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV298_RS25285	56323..57259	-	937	Protein_50	ISNCY family transposase	-
HV298_RS25290	57369..57539	+	171	Protein_51	transposase	-
HV298_RS25295	57537..57644	+	108	Protein_52	IS3 family transposase	-
HV298_RS25300	57878..58582	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
HV298_RS25305	58742..60088	-	1347	WP_077251107.1	ISNCY family transposase	-
HV298_RS25310	60299..60781	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
HV298_RS25315	60769..61035	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HV298_RS25320	61186..61890	+	705	WP_060579418.1	IS6-like element IS26 family transposase	-
HV298_RS25325	62049..64634	-	2586	WP_004187040.1	EAL domain-containing protein	-
HV298_RS25330	64603..64848	-	246	WP_032238678.1	hypothetical protein	-
HV298_RS25335	64906..65886	-	981	Protein_60	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131105	131105
	-	inside	IScluster/Tn	-	-	56302..65886	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T163228 WP_004187044.1 NZ_CP056298:c60781-60299 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T163228 NZ_CP072460:5022240-5022650 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT163228 WP_003026799.1 NZ_CP056298:c61035-60769 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163228 NZ_CP072460:5021993-5022259 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66