Detailed information of TA system TA163213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1743617..1744207
• Replicon: chromosome
• Accession: NZ_CP056297
• Organism: Klebsiella pneumoniae strain RHBSTW-00852

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV298_RS08370
• Protein ID: WP_021466317.1
• Coordinates: 1743875..1744207 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: HV298_RS08365
• Protein ID: WP_000288812.1
• Coordinates: 1743617..1743874 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV298_RS08350	1740960..1741739	-	780	WP_048291202.1	hypothetical protein	-
HV298_RS08355	1742607..1743079	+	473	Protein_1632	hypothetical protein	-
HV298_RS08360	1743076..1743282	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
HV298_RS08365	1743617..1743874	+	258	WP_000288812.1	antitoxin	Antitoxin
HV298_RS08370	1743875..1744207	+	333	WP_021466317.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV298_RS08380	1744530..1745966	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HV298_RS08390	1746332..1747786	-	1455	WP_004148975.1	AMP nucleosidase	-
HV298_RS08395	1747916..1748161	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1733870..1744207	10337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11899.82 Da
• Isoelectric Point: 10.4722

>T163213 WP_021466317.1 NZ_CP056297:1743875-1744207 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDAVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T163213 NZ_CP072456:c5341774-5341391 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT163213 WP_000288812.1 NZ_CP056297:1743617-1743874 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT163213 NZ_CP072456:c5342016-5341774 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0