Detailed information of TA system TA163209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 39886..40529
• Replicon: plasmid pRHBSTW-00867_3
• Accession: NZ_CP056277
• Organism: Klebsiella pneumoniae strain RHBSTW-00867

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV307_RS26830
• Protein ID: WP_101968530.1
• Coordinates: 40113..40529 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HV307_RS26825
• Protein ID: WP_001261276.1
• Coordinates: 39886..40116 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV307_RS26810	35885..36799	+	915	WP_101968528.1	Abi family protein	-
HV307_RS26815	37139..37624	+	486	WP_048263657.1	hypothetical protein	-
HV307_RS26820	37635..39638	+	2004	WP_101968529.1	AAA family ATPase	-
HV307_RS26825	39886..40116	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV307_RS26830	40113..40529	+	417	WP_101968530.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV307_RS26835	40699..41718	-	1020	Protein_38	AAA family ATPase	-
HV307_RS26840	41752..43341	-	1590	WP_015632444.1	IS66 family transposase	-
HV307_RS26845	43371..43721	-	351	WP_032414478.1	IS66 family insertion sequence element accessory protein TnpB	-
HV307_RS26850	43718..44125	-	408	WP_015632445.1	transposase	-
HV307_RS26855	44417..44842	+	426	WP_001776124.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51057	51057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15094.58 Da
• Isoelectric Point: 8.5403

>T163209 WP_101968530.1 NZ_CP056277:40113-40529 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPASVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T163209 NZ_CP072456:c4725928-4725395 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT163209 WP_001261276.1 NZ_CP056277:39886-40116 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT163209 NZ_CP072456:c4726205-4725939 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023