Detailed information of TA system TA163208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 8473..8995
• Replicon: plasmid pRHBSTW-00867_3
• Accession: NZ_CP056277
• Organism: Klebsiella pneumoniae strain RHBSTW-00867

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: HV307_RS26680
• Protein ID: WP_101968331.1
• Coordinates: 8473..8757 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HV307_RS26685
• Protein ID: WP_004187025.1
• Coordinates: 8747..8995 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV307_RS26660	4213..5820	+	1608	WP_101968519.1	EAL domain-containing protein	-
HV307_RS26665	6155..7302	+	1148	WP_181475417.1	IS3 family transposase	-
HV307_RS26670	7320..7460	-	141	Protein_5	IS1 family transposase	-
HV307_RS26675	7579..8237	-	659	Protein_6	IS1 family transposase	-
HV307_RS26680	8473..8757	-	285	WP_101968331.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV307_RS26685	8747..8995	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HV307_RS26690	9240..10163	-	924	WP_181475418.1	IS5 family transposase	-
HV307_RS26695	10387..11985	+	1599	WP_101968330.1	sensor domain-containing diguanylate cyclase	-
HV307_RS26700	12444..13577	+	1134	WP_101998594.1	glutathione-independent formaldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51057	51057
	-	inside	IScluster/Tn	-	-	2892..16741	13849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11020.77 Da
• Isoelectric Point: 10.4597

>T163208 WP_101968331.1 NZ_CP056277:c8757-8473 [Klebsiella pneumoniae]
MTYKLAFNESALKEWMKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGIGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T163208 NZ_CP072456:4115675-4115893 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT163208 WP_004187025.1 NZ_CP056277:c8995-8747 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT163208 NZ_CP072456:4115274-4115648 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4