Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3248..3838 Replicon plasmid pRHBSTW-00902_4
Accession NZ_CP056259
Organism Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag HV319_RS26620 Protein ID WP_003836692.1
Coordinates 3248..3580 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag HV319_RS26625 Protein ID WP_003836694.1
Coordinates 3581..3838 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV319_RS26605 804..1754 - 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
HV319_RS26610 1856..2773 - 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
HV319_RS26620 3248..3580 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HV319_RS26625 3581..3838 - 258 WP_003836694.1 hypothetical protein Antitoxin
HV319_RS26630 4562..5259 + 698 Protein_5 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - iutA 1..82097 82097
- flank IS/Tn - - 4966..5259 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T163167 WP_003836692.1 NZ_CP056259:c3580-3248 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T163167 NZ_CP072422:c3871406-3871089 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT163167 WP_003836694.1 NZ_CP056259:c3838-3581 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT163167 NZ_CP072422:c3871096-3870809 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References