Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 143962..144765 Replicon plasmid pRHBSTW-00902_2
Accession NZ_CP056257
Organism Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag HV319_RS25590 Protein ID WP_022652313.1
Coordinates 144235..144765 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag HV319_RS25585 Protein ID WP_022652312.1
Coordinates 143962..144231 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV319_RS25570 140267..140574 - 308 Protein_144 IS1 family transposase -
HV319_RS25575 141051..141791 + 741 WP_022652310.1 site-specific integrase -
HV319_RS25580 142117..143106 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
HV319_RS25585 143962..144231 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
HV319_RS25590 144235..144765 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
HV319_RS25595 144897..145790 + 894 WP_161348953.1 IS5 family transposase -
HV319_RS25600 145931..146407 - 477 Protein_150 IS3 family transposase -
HV319_RS25605 146488..147996 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
HV319_RS25610 148669..149098 - 430 Protein_152 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..206605 206605
- inside IScluster/Tn - - 138953..152159 13206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T163165 WP_022652313.1 NZ_CP056257:144235-144765 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T163165 NZ_CP072422:1943718-1943820 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT163165 WP_022652312.1 NZ_CP056257:143962-144231 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT163165 NZ_CP072422:c1943827-1943682 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References