Detailed information of TA system TA163164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 73812..74455
• Replicon: plasmid pRHBSTW-00902_2
• Accession: NZ_CP056257
• Organism: Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV319_RS25180
• Protein ID: WP_181502632.1
• Coordinates: 73812..74228 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6S5K7T9
• Locus tag: HV319_RS25185
• Protein ID: WP_059362947.1
• Coordinates: 74225..74455 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV319_RS25165	68823..69827	+	1005	WP_117273198.1	IS110-like element IS4321 family transposase	-
HV319_RS25170	70173..71013	+	841	Protein_64	EAL domain-containing protein	-
HV319_RS25175	72166..73110	-	945	WP_181502633.1	sensor domain-containing diguanylate cyclase	-
HV319_RS25180	73812..74228	-	417	WP_181502632.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV319_RS25185	74225..74455	-	231	WP_059362947.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV319_RS25190	75009..75359	+	351	WP_009654828.1	hypothetical protein	-
HV319_RS25195	75399..76166	+	768	WP_009654830.1	hypothetical protein	-
HV319_RS25200	76169..76945	+	777	WP_032426116.1	site-specific integrase	-
HV319_RS25205	77090..78079	-	990	WP_020804955.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..206605	206605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15053.49 Da
• Isoelectric Point: 7.8637

>T163164 WP_181502632.1 NZ_CP056257:c74228-73812 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDAATDIKVALRLAGTPIGPNDTLIAGHAIAAGAVLVTRDGGEFERVPGLTLEDWAR

Nucleotide

• Length: 417 bp

>T163164 NZ_CP072422:782466-782876 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8798.87 Da
• Isoelectric Point: 4.2917

>AT163164 WP_059362947.1 NZ_CP056257:c74455-74225 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFFELEKADPDFMAEREDVVTDEGRFEP

Nucleotide

• Length: 231 bp

>AT163164 NZ_CP072422:782219-782485 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6S5K7T9