Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3739502..3740122 Replicon chromosome
Accession NZ_CP056256
Organism Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV319_RS18245 Protein ID WP_002892050.1
Coordinates 3739904..3740122 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV319_RS18240 Protein ID WP_003021733.1
Coordinates 3739502..3739876 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV319_RS18230 3734648..3735841 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV319_RS18235 3735864..3739013 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV319_RS18240 3739502..3739876 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV319_RS18245 3739904..3740122 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV319_RS18250 3740429..3740980 + 552 WP_096878255.1 maltose O-acetyltransferase -
HV319_RS18255 3741097..3741567 + 471 WP_003021724.1 YlaC family protein -
HV319_RS18260 3741646..3741786 - 141 WP_181501926.1 type B 50S ribosomal protein L36 -
HV319_RS18265 3741788..3742048 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HV319_RS18270 3742237..3743790 + 1554 WP_003835927.1 EAL domain-containing protein -
HV319_RS18275 3743842..3744195 - 354 WP_103848454.1 DUF1428 family protein -
HV319_RS18280 3744260..3744889 - 630 WP_016149709.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163160 WP_002892050.1 NZ_CP056256:3739904-3740122 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163160 NZ_CP072413:c5269518-5269135 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163160 WP_003021733.1 NZ_CP056256:3739502-3739876 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163160 NZ_CP072413:c5269760-5269518 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References