Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 934341..935113 Replicon chromosome
Accession NZ_CP056256
Organism Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2I8S4E2
Locus tag HV319_RS04565 Protein ID WP_065805889.1
Coordinates 934724..935113 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID -
Locus tag HV319_RS04560 Protein ID WP_171820947.1
Coordinates 934341..934667 (+) Length 109 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV319_RS04530 930786..931418 - 633 WP_072032909.1 inovirus Gp2 family protein -
HV319_RS04535 931956..932780 + 825 WP_065805884.1 DUF945 domain-containing protein -
HV319_RS04540 932777..933034 + 258 WP_044349282.1 DUF905 domain-containing protein -
HV319_RS04545 933114..933557 + 444 WP_065805885.1 antirestriction protein -
HV319_RS04550 933569..934048 + 480 WP_128301357.1 DNA repair protein RadC -
HV319_RS04555 934062..934283 + 222 WP_053895773.1 DUF987 domain-containing protein -
HV319_RS04560 934341..934667 + 327 WP_171820947.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV319_RS04565 934724..935113 + 390 WP_065805889.1 TA system toxin CbtA family protein Toxin
HV319_RS04570 935200..936039 + 840 WP_128301359.1 DUF4942 domain-containing protein -
HV319_RS04575 936777..936998 + 222 WP_003846405.1 hypothetical protein -
HV319_RS04580 937035..937667 + 633 WP_003838326.1 hypothetical protein -
HV319_RS04585 937751..938020 - 270 WP_064375540.1 hypothetical protein -
HV319_RS04590 938096..938404 - 309 WP_170864030.1 hypothetical protein -
HV319_RS04595 938386..938583 - 198 WP_044700790.1 hypothetical protein -
HV319_RS04600 938677..939255 - 579 WP_061549908.1 tyrosine-type recombinase/integrase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 905515..947183 41668


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-111)

Antitoxin

(4-103)


Sequences


Toxin        


Download         Length: 130 a.a.        Molecular weight: 14321.40 Da        Isoelectric Point: 9.9883

>T163151 WP_065805889.1 NZ_CP056256:934724-935113 [Citrobacter freundii]
MQTKSSPLMRAASSRPSPVSVWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Download         Length: 390 bp

>T163151 NZ_CP072408:c5278110-5277727 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12113.85 Da        Isoelectric Point: 6.9554

>AT163151 WP_171820947.1 NZ_CP056256:934341-934667 [Citrobacter freundii]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHGIILYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Download         Length: 327 bp

>AT163151 NZ_CP072408:c5278352-5278110 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2I8S4E2


Antitoxin

Source ID Structure

References