Detailed information of TA system TA163149

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 775728..776483
• Replicon: chromosome
• Accession: NZ_CP056256
• Organism: Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ILI8
• Locus tag: HV319_RS03760
• Protein ID: WP_047359063.1
• Coordinates: 775728..776105 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8I0KKX4
• Locus tag: HV319_RS03765
• Protein ID: WP_073970862.1
• Coordinates: 776157..776483 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV319_RS03740	771750..773609	+	1860	WP_003838200.1	bifunctional glutathionylspermidine amidase/synthase	-
HV319_RS03745	774081..774929	-	849	WP_088732394.1	DUF4942 domain-containing protein	-
HV319_RS03750	775008..775211	-	204	WP_047359061.1	DUF957 domain-containing protein	-
HV319_RS03755	775240..775731	-	492	WP_047359062.1	hypothetical protein	-
HV319_RS03760	775728..776105	-	378	WP_047359063.1	TA system toxin CbtA family protein	Toxin
HV319_RS03765	776157..776483	-	327	WP_073970862.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV319_RS03770	776540..776761	-	222	WP_000691982.1	DUF987 domain-containing protein	-
HV319_RS03775	776782..777261	-	480	WP_047359065.1	DNA repair protein RadC	-
HV319_RS03780	777273..777734	-	462	WP_047359066.1	antirestriction protein	-
HV319_RS03785	777831..778070	-	240	WP_047359067.1	DUF905 domain-containing protein	-
HV319_RS03790	778148..778621	-	474	WP_181502091.1	hypothetical protein	-
HV319_RS03795	778643..779338	-	696	WP_047359068.1	hypothetical protein	-
HV319_RS03800	779521..780222	-	702	WP_047359069.1	WYL domain-containing protein	-
HV319_RS03805	780438..781259	-	822	WP_181502092.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14078.86 Da
• Isoelectric Point: 7.0606

>T163149 WP_047359063.1 NZ_CP056256:c776105-775728 [Citrobacter freundii]
VQTQPLSSTHETSPRPSPVDIWQHLLNHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
SADTQSPLIGSIDILRARKATGLMTRHGYRPVTDLITGKYKKEQQ

Nucleotide

• Length: 378 bp

>T163149 NZ_CP072408:c4660904-4660371 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11969.65 Da
• Isoelectric Point: 6.9554

>AT163149 WP_073970862.1 NZ_CP056256:c776483-776157 [Citrobacter freundii]
ITEPWWGLKRSITPCFGARLVQEGNRLHYLADRASIAGTFSDADLRHLDQAFPVQLKQLELMLVSGELNPRHQHCVTLYS
KGLTCEADSLGSHGYVYIAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT163149 NZ_CP072408:c4661181-4660915 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6ILI8