Detailed information of TA system TA163148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 299559..300145
• Replicon: chromosome
• Accession: NZ_CP056256
• Organism: Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0J1QS94
• Locus tag: HV319_RS01420
• Protein ID: WP_032937237.1
• Coordinates: 299777..300145 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0J1N092
• Locus tag: HV319_RS01415
• Protein ID: WP_032937236.1
• Coordinates: 299559..299780 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV319_RS01390	295358..296284	+	927	WP_003023435.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HV319_RS01395	296281..297558	+	1278	WP_103848242.1	branched chain amino acid ABC transporter permease LivM	-
HV319_RS01400	297555..298322	+	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HV319_RS01405	298340..299053	+	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HV319_RS01410	299156..299437	+	282	WP_003023442.1	hypothetical protein	-
HV319_RS01415	299559..299780	+	222	WP_032937236.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV319_RS01420	299777..300145	+	369	WP_032937237.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV319_RS01425	300389..301705	+	1317	WP_003023445.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HV319_RS01430	301806..302693	+	888	WP_103848245.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HV319_RS01435	302690..303535	+	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HV319_RS01440	303538..304608	+	1071	WP_003837845.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	294187..305348	11161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.91 Da
• Isoelectric Point: 6.9891

>T163148 WP_032937237.1 NZ_CP056256:299777-300145 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Nucleotide

• Length: 369 bp

>T163148 NZ_CP072408:4057281-4057499 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8306.41 Da
• Isoelectric Point: 4.7906

>AT163148 WP_032937236.1 NZ_CP056256:299559-299780 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT163148 NZ_CP072408:4056880-4057254 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0J1QS94

Antitoxin

• AlphaFold DB: A0A0J1N092