Detailed information of TA system TA163142

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4211867..4212546
• Replicon: chromosome
• Accession: NZ_CP056251
• Organism: Citrobacter sp. RHBSTW-00903

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2I8S2T2
• Locus tag: HV320_RS21000
• Protein ID: WP_008324514.1
• Coordinates: 4212205..4212546 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A2I8S2U6
• Locus tag: HV320_RS20995
• Protein ID: WP_008324516.1
• Coordinates: 4211867..4212184 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV320_RS20950	4207308..4208129	+	822	WP_032654809.1	DUF945 domain-containing protein	-
HV320_RS20955	4208347..4209048	+	702	WP_008324530.1	WYL domain-containing protein	-
HV320_RS20960	4209085..4209321	+	237	WP_001115854.1	hypothetical protein	-
HV320_RS20965	4209321..4209764	+	444	WP_000824223.1	hypothetical protein	-
HV320_RS20970	4209787..4210254	+	468	WP_001547765.1	hypothetical protein	-
HV320_RS20975	4210331..4210570	+	240	WP_000194654.1	DUF905 domain-containing protein	-
HV320_RS20980	4210668..4211126	+	459	WP_000211838.1	antirestriction protein	-
HV320_RS20985	4211142..4211618	+	477	WP_000811693.1	RadC family protein	-
HV320_RS20990	4211627..4211854	+	228	WP_008324517.1	DUF987 domain-containing protein	-
HV320_RS20995	4211867..4212184	+	318	WP_008324516.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV320_RS21000	4212205..4212546	+	342	WP_008324514.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HV320_RS21005	4212752..4213872	+	1121	WP_103015411.1	IS3 family transposase	-
HV320_RS21015	4214394..4214858	-	465	WP_103283511.1	isoprenylcysteine carboxylmethyltransferase family protein	-
HV320_RS21020	4215052..4216500	-	1449	WP_103283510.1	EAL domain-containing protein	-
HV320_RS21025	4216843..4217316	-	474	WP_016155774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4187023..4213872	26849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12919.98 Da
• Isoelectric Point: 9.6543

>T163142 WP_008324514.1 NZ_CP056251:4212205-4212546 [Citrobacter sp. RHBSTW-00903]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T163142 NZ_CP072403:c5278268-5277885 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11787.33 Da
• Isoelectric Point: 7.3476

>AT163142 WP_008324516.1 NZ_CP056251:4211867-4212184 [Citrobacter sp. RHBSTW-00903]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT163142 NZ_CP072403:c5278510-5278268 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A2I8S2T2

Antitoxin

• AlphaFold DB: A0A2I8S2U6