Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4023644..4024264 Replicon chromosome
Accession NZ_CP056251
Organism Citrobacter sp. RHBSTW-00903

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV320_RS20120 Protein ID WP_002892050.1
Coordinates 4024046..4024264 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV320_RS20115 Protein ID WP_003021733.1
Coordinates 4023644..4024018 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV320_RS20105 4018791..4019984 + 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV320_RS20110 4020007..4023156 + 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV320_RS20115 4023644..4024018 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV320_RS20120 4024046..4024264 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV320_RS20125 4024445..4024996 + 552 WP_103283555.1 maltose O-acetyltransferase -
HV320_RS20130 4025113..4025583 + 471 WP_016152071.1 YlaC family protein -
HV320_RS20135 4025661..4025801 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV320_RS20140 4025803..4026063 - 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV320_RS20145 4026252..4027805 + 1554 WP_047416867.1 EAL domain-containing protein -
HV320_RS20150 4027857..4028210 - 354 WP_003831033.1 DUF1428 family protein -
HV320_RS20155 4028275..4028904 - 630 WP_103283554.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163141 WP_002892050.1 NZ_CP056251:4024046-4024264 [Citrobacter sp. RHBSTW-00903]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163141 NZ_CP072403:c4793054-4792770 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163141 WP_003021733.1 NZ_CP056251:4023644-4024018 [Citrobacter sp. RHBSTW-00903]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163141 NZ_CP072403:c4793286-4793044 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References