Detailed information of TA system TA163139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2883457..2884093
• Replicon: chromosome
• Accession: NZ_CP056251
• Organism: Citrobacter sp. RHBSTW-00903

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV320_RS14610
• Protein ID: WP_049259794.1
• Coordinates: 2883457..2883645 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV320_RS14615
• Protein ID: WP_146053235.1
• Coordinates: 2883677..2884093 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV320_RS14590	2880235..2880465	-	231	WP_016156585.1	DUF2554 family protein	-
HV320_RS14595	2880655..2880780	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV320_RS14600	2880780..2881790	-	1011	WP_103283796.1	cytochrome d ubiquinol oxidase subunit II	-
HV320_RS14605	2881790..2883193	-	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
HV320_RS14610	2883457..2883645	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV320_RS14615	2883677..2884093	+	417	WP_146053235.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV320_RS14620	2884186..2885595	+	1410	WP_103283798.1	PLP-dependent aminotransferase family protein	-
HV320_RS14625	2885925..2887070	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV320_RS14630	2887087..2888103	+	1017	WP_016153153.1	ABC transporter ATP-binding protein	-
HV320_RS14635	2888104..2889048	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T163139 WP_049259794.1 NZ_CP056251:2883457-2883645 [Citrobacter sp. RHBSTW-00903]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T163139 NZ_CP072403:4057439-4057657 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.33 Da
• Isoelectric Point: 4.7119

>AT163139 WP_146053235.1 NZ_CP056251:2883677-2884093 [Citrobacter sp. RHBSTW-00903]
MRYPVNLIPSVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT163139 NZ_CP072403:4057038-4057412 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9