Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 154734..155260 Replicon plasmid pRHBSTW-00915_2
Accession NZ_CP056246
Organism Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV325_RS25005 Protein ID WP_000323025.1
Coordinates 154973..155260 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV325_RS25000 Protein ID WP_000534858.1
Coordinates 154734..154973 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV325_RS24985 151062..152300 + 1239 WP_003030308.1 IS110 family transposase -
HV325_RS24990 152776..153348 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HV325_RS24995 153548..154471 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
HV325_RS25000 154734..154973 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV325_RS25005 154973..155260 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV325_RS25010 155358..156017 + 660 WP_008786574.1 hypothetical protein -
HV325_RS25015 156066..156311 + 246 WP_007372330.1 hypothetical protein -
HV325_RS25020 156515..159892 + 3378 WP_102016262.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..178786 178786


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T163128 WP_000323025.1 NZ_CP056246:154973-155260 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T163128 NZ_CP072396:c4545773-4545621 [Xanthomonas citri pv. fuscans]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT163128 WP_000534858.1 NZ_CP056246:154734-154973 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT163128 NZ_CP072396:c4545546-4545412 [Xanthomonas citri pv. fuscans]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References