Detailed information of TA system TA163127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 125154..126055
• Replicon: plasmid pRHBSTW-00915_2
• Accession: NZ_CP056246
• Organism: Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: HV325_RS24830
• Protein ID: WP_008786589.1
• Coordinates: 125154..125612 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV325_RS24835
• Protein ID: WP_008786588.1
• Coordinates: 125609..126055 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV325_RS24790	120499..120999	-	501	WP_001752509.1	non-heme ferritin-like protein	-
HV325_RS24795	121326..122030	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HV325_RS24800	122304..122642	-	339	WP_007372294.1	hypothetical protein	-
HV325_RS24805	122880..123323	+	444	WP_007372295.1	hypothetical protein	-
HV325_RS24810	123442..123783	+	342	WP_007372296.1	hypothetical protein	-
HV325_RS24815	123942..124496	+	555	WP_007372297.1	hypothetical protein	-
HV325_RS24820	124546..124812	+	267	WP_007372298.1	hypothetical protein	-
HV325_RS24825	124880..125110	-	231	WP_007372299.1	hypothetical protein	-
HV325_RS24830	125154..125612	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
HV325_RS24835	125609..126055	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HV325_RS24840	126204..126521	+	318	WP_016241555.1	hypothetical protein	-
HV325_RS24845	126563..127405	+	843	WP_007372303.1	hypothetical protein	-
HV325_RS24850	127377..127655	+	279	WP_007372304.1	hypothetical protein	-
HV325_RS24855	127715..128158	+	444	WP_007372305.1	hypothetical protein	-
HV325_RS24860	128445..128609	+	165	WP_007372306.1	hypothetical protein	-
HV325_RS24865	129177..130019	+	843	WP_007372307.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..178786	178786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T163127 WP_008786589.1 NZ_CP056246:c125612-125154 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T163127 NZ_CP072396:c4545546-4545412 [Xanthomonas citri pv. fuscans]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT163127 WP_008786588.1 NZ_CP056246:c126055-125609 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT163127 NZ_CP072396:c4545773-4545621 [Xanthomonas citri pv. fuscans]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: S1EZ83