Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3666724..3667344 Replicon chromosome
Accession NZ_CP056245
Organism Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV325_RS18060 Protein ID WP_002892050.1
Coordinates 3667126..3667344 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV325_RS18055 Protein ID WP_003021733.1
Coordinates 3666724..3667098 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV325_RS18045 3661870..3663063 + 1194 WP_049015706.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV325_RS18050 3663086..3666235 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV325_RS18055 3666724..3667098 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV325_RS18060 3667126..3667344 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV325_RS18065 3667526..3668077 + 552 WP_049015707.1 maltose O-acetyltransferase -
HV325_RS18070 3668194..3668664 + 471 WP_003021724.1 YlaC family protein -
HV325_RS18075 3668743..3668883 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV325_RS18080 3668885..3669145 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HV325_RS18085 3669334..3670887 + 1554 WP_003835927.1 EAL domain-containing protein -
HV325_RS18090 3670939..3671292 - 354 WP_003831033.1 DUF1428 family protein -
HV325_RS18095 3671357..3671986 - 630 WP_049015708.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163123 WP_002892050.1 NZ_CP056245:3667126-3667344 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163123 NZ_CP072393:c4994454-4994341 [Xanthomonas phaseoli pv. phaseoli]
TTGAAGGGCGAGCGGCGCGGCCAGTACCGCATCCGGATCAACGATCGATGGCGCATCTGCGTCCGATGGATGGAAGGCGA
TGTGGTCCAGGTCGAAATCATCGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163123 WP_003021733.1 NZ_CP056245:3666724-3667098 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163123 NZ_CP072393:c4994323-4994033 [Xanthomonas phaseoli pv. phaseoli]
ATGACGGTCCTGCCCAATATCCACCCTGGCGCAATTCTGCTTGAGGAGTTTCTCCAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGAACCTTCGAGCGCTTCTGGCTTGGCCTGCAGGCCGACGACGAGCTGGAGCAGGCG
CATCGCGCGCTGGGCGACTTGCCGGCGCGGATCAAGCGGCTGGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References