Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2753288..2753814 Replicon chromosome
Accession NZ_CP056245
Organism Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV325_RS13520 Protein ID WP_000323025.1
Coordinates 2753288..2753575 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV325_RS13525 Protein ID WP_000534858.1
Coordinates 2753575..2753814 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV325_RS13495 2748915..2749466 + 552 WP_003029269.1 hypothetical protein -
HV325_RS13500 2749471..2750253 + 783 WP_032941311.1 DUF1120 domain-containing protein -
HV325_RS13505 2750326..2751306 + 981 WP_003029273.1 helix-turn-helix domain-containing protein -
HV325_RS13510 2751385..2752395 + 1011 WP_016150174.1 type I glyceraldehyde-3-phosphate dehydrogenase -
HV325_RS13515 2752608..2753085 + 478 Protein_2649 glutathione peroxidase -
HV325_RS13520 2753288..2753575 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV325_RS13525 2753575..2753814 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV325_RS13530 2754077..2755000 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
HV325_RS13535 2755200..2755772 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HV325_RS13540 2756248..2757486 - 1239 WP_003030308.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2742688..2755772 13084


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T163122 WP_000323025.1 NZ_CP056245:c2753575-2753288 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T163122 NZ_CP072393:c4451885-4451733 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGTCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAGTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT163122 WP_000534858.1 NZ_CP056245:c2753814-2753575 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT163122 NZ_CP072393:c4451658-4451524 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References