Detailed information of TA system TA163120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2503635..2504274
• Replicon: chromosome
• Accession: NZ_CP056245
• Organism: Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV325_RS12330
• Protein ID: WP_003020221.1
• Coordinates: 2503635..2503811 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV325_RS12335
• Protein ID: WP_123924900.1
• Coordinates: 2503858..2504274 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV325_RS12310	2500416..2500646	-	231	WP_003020233.1	DUF2554 family protein	-
HV325_RS12315	2500836..2500961	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV325_RS12320	2500961..2501971	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
HV325_RS12325	2501971..2503374	-	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
HV325_RS12330	2503635..2503811	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV325_RS12335	2503858..2504274	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV325_RS12340	2504367..2505776	+	1410	WP_003843728.1	PLP-dependent aminotransferase family protein	-
HV325_RS12345	2506105..2507250	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
HV325_RS12350	2507267..2508283	+	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
HV325_RS12355	2508284..2509228	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T163120 WP_003020221.1 NZ_CP056245:2503635-2503811 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T163120 NZ_CP072387:c3652393-3652115 [Cellulosimicrobium cellulans]
GTGACCGACGACCAGACACCGGCGTGGCGGGTCGAGATCGCACCCGCGGCCGAGCGGTCGCTGCTGCGCCTGCCGCACAA
GATCGCGGCCGCGGTCGTCGAGTTCGTCACCACGACCCTGCCGACCAACCCCGAACGCATGAGCAAGCCGCTGCGGTACG
AGCTCGAAGGCCTGCGCTCCGCGCGCCGCGGCGACTACCGCGTGCTGTTCGAGCTCGACCACGACACCGGGGTGCTGCTC
GTCGTCCGCATCGCGCACCGCGCCGACGCCTACCGATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT163120 WP_123924900.1 NZ_CP056245:2503858-2504274 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT163120 NZ_CP072387:c3652665-3652390 [Cellulosimicrobium cellulans]
ATGAAATCCGTAGCGCTGAGCGAGGCGAAAGACAAGTTGTCGGCCCTGGTGGACGAGGCCGACCGTGAGCACGAGATCAT
CCGGATCACCCGGCACGGGCGCGGTGCCGCGGTGCTGATGGCCGAGGACGACCTCGAGTCGCTGCGCGAGACGCTGTTCT
GGCTGTCCCAGCCCGGCGTGCGCGACGACATCGACGAGGCCCGTCGCGCCGCCGCCGAGGGCGCCACGACGTCCGGTGAG
GACCTGCGGGCACGGTTCGGGCTGCCGGCGCAGTGA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36