Detailed information of TA system TA163110

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 896239..896900
• Replicon: chromosome
• Accession: NZ_CP056245
• Organism: Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV325_RS04460
• Protein ID: WP_048997879.1
• Coordinates: 896577..896900 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A241Q747
• Locus tag: HV325_RS04455
• Protein ID: WP_048997880.1
• Coordinates: 896239..896556 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV325_RS04415	892230..892940	+	711	WP_048998192.1	DeoR family transcriptional regulator	-
HV325_RS04420	892966..893502	+	537	WP_048998191.1	DUF4339 domain-containing protein	-
HV325_RS04425	893554..894120	+	567	WP_048998190.1	hypothetical protein	-
HV325_RS04430	894210..894620	+	411	WP_048998189.1	hypothetical protein	-
HV325_RS04435	894698..894937	+	240	WP_048998188.1	DUF905 domain-containing protein	-
HV325_RS04440	895040..895498	+	459	WP_049016518.1	antirestriction protein	-
HV325_RS04445	895514..895990	+	477	WP_049016517.1	RadC family protein	-
HV325_RS04450	895999..896220	+	222	WP_048997881.1	DUF987 domain-containing protein	-
HV325_RS04455	896239..896556	+	318	WP_048997880.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV325_RS04460	896577..896900	+	324	WP_048997879.1	TA system toxin CbtA family protein	Toxin
HV325_RS04465	897141..897677	-	537	WP_044699787.1	GNAT family N-acetyltransferase	-
HV325_RS04470	898494..900956	+	2463	WP_044713448.1	poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	840460..896900	56440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12455.32 Da
• Isoelectric Point: 6.4624

>T163110 WP_048997879.1 NZ_CP056245:896577-896900 [Citrobacter freundii]
MKSQPATTQRAAKPCLSPVDVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T163110 NZ_CP072385:2924090-2924338 [Arachnia propionica]
ATGTCGTACACCGAGTCCCGAGCCAACTACGCGGCGGTCCTCGACGAGGTCGTGAACAATCGCGAGGAGATCGTCATCAC
CCGGGCAGGACACGAACCCGTCGTGATCGTCTCCCTGGAGGAGTACCAGTCCCTTCGCGAGACCGCCTACCTGTTGCGTT
CGCCCGCCAACGCCCAGCGGCTGGCCAGCGCCATGGAACAGCTCGACAGGAACGACGGCATCCCGCACGACCTCCTCGAC
GACCACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11993.64 Da
• Isoelectric Point: 7.6976

>AT163110 WP_048997880.1 NZ_CP056245:896239-896556 [Citrobacter freundii]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGQFSDAECRKLDVAFPYFIRQMESMLTTGELNPRHTHCVTLYR
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT163110 NZ_CP072385:2924340-2924600 [Arachnia propionica]
ATGCGGATCTGCTGGGCCGACGAAGCGTGGGAGGACTACCTCTGGTGGCAAACCCAGGACCGCCGGACCCTCAAGAGAAT
CAACTCCCTGATCCGGGACATCGCTCGCAACGGAAACGAGGAACTGGGCAAACCGGAGGCGCTGCGATACCAACTCTCCG
GCTACTGGTCCCGGCGTATCACTGAGGAGCATCGGCTGGTCTACCGAATCACCGACACCGAGGTCCAGATTGCCGCATGC
CGGTACCACTATGGCCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A241Q747