Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 815793..816447 Replicon chromosome
Accession NZ_CP056245
Organism Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag HV325_RS04040 Protein ID WP_003026936.1
Coordinates 816040..816447 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag HV325_RS04035 Protein ID WP_003026938.1
Coordinates 815793..816059 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV325_RS04010 810994..812427 - 1434 WP_049015307.1 6-phospho-beta-glucosidase BglA -
HV325_RS04015 812548..813276 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
HV325_RS04020 813329..813640 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
HV325_RS04025 813803..814462 + 660 WP_003838267.1 hemolysin III family protein -
HV325_RS04030 814556..815536 - 981 WP_003026944.1 tRNA-modifying protein YgfZ -
HV325_RS04035 815793..816059 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
HV325_RS04040 816040..816447 + 408 WP_003026936.1 protein YgfX Toxin
HV325_RS04045 816492..817013 - 522 WP_003026933.1 flavodoxin FldB -
HV325_RS04050 817127..818023 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
HV325_RS04055 818052..818765 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HV325_RS04060 818771..820504 + 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T163109 WP_003026936.1 NZ_CP056245:816040-816447 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T163109 NZ_CP072385:1291411-1291758 [Arachnia propionica]
GTGCTCCGCGGCGAGATCCGGCTGGTCGATCTGGAGCCCGCACGGGCGGGAGAGGCAGACAAGCGTCGTCCGGCGATCAT
CGTCAGCAACAACCGCGCCAACACCGTCGCCGCTCGACTCGGGCGAGGCGTGGTCACGGTCGTCCCCGTCACCAGTAATA
CCGCGCGGGTCTTTCCGTTTCAGGTGCTTCTCACCGCGGATGAGGTCGGCATCCGCTTGGATTCCAAGGCCCAGGCCGAA
CAGGTCCGAGCGGTGTCAGTCGACCGCATCGGCCCGGTGATCGGGCGCCTTCCCGCCCGCCTGGTCGCCCAACTGGATGA
GGCGCTCCGGCTGCACCTTCAGCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT163109 WP_003026938.1 NZ_CP056245:815793-816059 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT163109 NZ_CP072385:1291184-1291411 [Arachnia propionica]
ATGGGTCAGGTGAAGTTGAGCGTGAGTCTGTCGCCGAGCGAGGTCGCCGTGCTCGACAAGTACGCACAGTCTGCGGGTTT
GAAGTCACGATCGGCGGCGATCCAACAGGCCATCAAGCTGCTGGGCGATCCCGACCTGGAGGATGCCTACACCGCTGCCT
GGCAGGAGTGGGAGGACGCCGGAGAGGCCGAGGCCTGGGCGGCCACCGTCGCCGACGGGGTGAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References