Detailed information of TA system TA163106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4489260..4489845
• Replicon: chromosome
• Accession: NZ_CP056244
• Organism: Citrobacter freundii strain RHBSTW-00923

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HV327_RS21265
• Protein ID: WP_135912739.1
• Coordinates: 4489260..4489574 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HV327_RS21270
• Protein ID: WP_115259723.1
• Coordinates: 4489567..4489845 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV327_RS21240	4485374..4486954	+	1581	WP_181517663.1	RNA repair transcriptional activator RtcR	-
HV327_RS21245	4487059..4487331	+	273	WP_096758747.1	DUF3811 domain-containing protein	-
HV327_RS21250	4487328..4488203	-	876	WP_096758748.1	23S rRNA pseudouridine(2604) synthase RluF	-
HV327_RS21255	4488454..4488771	-	318	WP_115259720.1	CcdB family protein	-
HV327_RS21260	4488771..4489064	-	294	WP_181517664.1	type II toxin-antitoxin system CcdA family antitoxin	-
HV327_RS21265	4489260..4489574	+	315	WP_135912739.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV327_RS21270	4489567..4489845	+	279	WP_115259723.1	putative addiction module antidote protein	Antitoxin
HV327_RS21275	4489957..4490646	+	690	WP_115259724.1	dipeptidase PepE	-
HV327_RS21280	4490775..4492406	-	1632	WP_117343624.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11969.88 Da
• Isoelectric Point: 9.5570

>T163106 WP_135912739.1 NZ_CP056244:4489260-4489574 [Citrobacter freundii]
MEILQTMVFQRWEQNLRDRRAKTLIATRLFRLANGLAGDVKPVGEGISEMRISYGPGYRIYFKQQGCHIIILLCGGDKSS
QANDITMAKMLARSLDYQEIRHHE

Nucleotide

• Length: 315 bp

>T163106 NZ_CP072385:618261-618515 [Arachnia propionica]
GTGCGCCTGGCGTGGGATCCCTCGGCCTGGGAGGATTACACGTACTGGCAGGCCGCGGATAGCCGGATTCTCAAGCGGAT
CAACGCGCTCATCGACACCTGCCTCCGCGATCCCTTCGGTGGGATCGGGAAACCTGAACAACTCAGGTATGGGGCAACGG
GGGCTTGGTCGCGGCGCATCACGGAGGAGCACCGGCTCGTGTATCTCGTCGACGGCGACGACCTGGTGATCCTCCAGGCC
CGGTACCACTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9863.44 Da
• Isoelectric Point: 6.3200

>AT163106 WP_115259723.1 NZ_CP056244:4489567-4489845 [Citrobacter freundii]
MNKLTRYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKALGIELTLKK

Nucleotide

• Length: 279 bp

>AT163106 NZ_CP072385:618008-618259 [Arachnia propionica]
GTGTCCATCAGCGCCAGCGAGGCCCGCAGGACGTTGTTCCCGCTCATCGAGCAGGTCAACGACGATCGCACTGCCGTCGA
GATCGTTTCCCGCAAGGGGAACGCCGTTCTCATGTCCGCCGATGAATACGCCGCGTGGCAGGAGACGGCCTACCTGTTCC
GTTCCCCCGCCAATGCCCGAAGGCTGCTCGACGCCTACGAGAGGGCCCGGGAAGGACGGAGCGAGGAACACGATCTCATC
CGGGAGGACTGA