Detailed information of TA system TA163104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3599336..3599956
• Replicon: chromosome
• Accession: NZ_CP056244
• Organism: Citrobacter freundii strain RHBSTW-00923

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV327_RS17105
• Protein ID: WP_002892050.1
• Coordinates: 3599738..3599956 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV327_RS17100
• Protein ID: WP_115259201.1
• Coordinates: 3599336..3599710 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV327_RS17090	3594482..3595675	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV327_RS17095	3595698..3598847	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HV327_RS17100	3599336..3599710	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HV327_RS17105	3599738..3599956	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV327_RS17110	3600334..3600807	+	474	WP_096758041.1	YlaC family protein	-
HV327_RS17115	3600881..3601021	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV327_RS17120	3601023..3601283	-	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HV327_RS17125	3601468..3603021	+	1554	WP_181517468.1	EAL domain-containing protein	-
HV327_RS17130	3603075..3603428	-	354	WP_115259217.1	DUF1428 family protein	-
HV327_RS17135	3603511..3604122	-	612	WP_181517469.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T163104 WP_002892050.1 NZ_CP056244:3599738-3599956 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T163104 NZ_CP072385:604580-604861 [Arachnia propionica]
ATGATCGTCTCCTTCGCCGATCGCGACGCTGAGCGACTGTTCCAAAGGCAGCGCGTCAAGCGCCTTGATCCTCGTCTCCA
CAGGAAGGCGCTCATCAAGTTGTGGTTGTTGGATGCCGTCCACGAACTCGACGAGCTTCGAGTGCCGCCGGGTAACCGGC
TGGAGGCGCTCAAGGGAGCCCGAATCGGTCAGCACAGCATCAGGATCAATGACCAGTGGCGGATATGTTTCGTGTGGACC
CCGGCGGGCCCGAGCGAAGTTGAGATCGTGGACTACCATTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT163104 WP_115259201.1 NZ_CP056244:3599336-3599710 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT163104 NZ_CP072385:604872-605171 [Arachnia propionica]
ATGGACAACAAAATGGCTCCGGTTCATCCAGGAGAAATTCTCGTGGAGGAGTTCCTGAATCCGATGGGCGTTACTCAGCA
TCGGCTCGCGATCTCCATTGGGGTGCCGCCCCGCCGAATCAACGAGATTGTGCACGGCAAGCGTCGGATCACGGCTGACA
CAGCATTGCGGCTGGGACGCTTCTTCGGAATGAATCCGCAATTCTGGGTCAACTTGCAATCGCACTACGATCTCGAAATC
GGGAAGGATGCACTGGGCGATGAGCTGGACAAGATTCAGCCTCTGAGATCTGTCGCATGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2