Detailed information of TA system TA163103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2403308..2404077
• Replicon: chromosome
• Accession: NZ_CP056244
• Organism: Citrobacter freundii strain RHBSTW-00923

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV327_RS11245
• Protein ID: WP_181518299.1
• Coordinates: 2403595..2404077 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HV327_RS11240
• Protein ID: WP_168246960.1
• Coordinates: 2403308..2403604 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV327_RS11225	2398808..2399995	+	1188	WP_146716827.1	outer membrane protein transport protein	-
HV327_RS11230	2400275..2400904	+	630	WP_181518298.1	winged helix-turn-helix transcriptional regulator	-
HV327_RS11235	2401213..2403195	+	1983	WP_117342783.1	MBL fold metallo-hydrolase	-
HV327_RS11240	2403308..2403604	+	297	WP_168246960.1	DUF1778 domain-containing protein	Antitoxin
HV327_RS11245	2403595..2404077	+	483	WP_181518299.1	GNAT family N-acetyltransferase	Toxin
HV327_RS11250	2404281..2405072	-	792	WP_181518300.1	SDR family oxidoreductase	-
HV327_RS11255	2405221..2406114	+	894	WP_137363300.1	LysR family transcriptional regulator	-
HV327_RS11260	2406149..2407138	-	990	WP_181518301.1	aldo/keto reductase	-
HV327_RS11265	2407165..2407455	-	291	Protein_2207	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (17-91)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17702.31 Da
• Isoelectric Point: 7.2887

>T163103 WP_181518299.1 NZ_CP056244:2403595-2404077 [Citrobacter freundii]
MEINVTAPALLTEGHELQPFDCGNEVLNDWLRRRAIKNQYLNASRTFVICLESTQCVVGYYSIATGSVSHADLGRSLRQN
MPDPVPVVLLGRLAVDASTQGHSYGKWLLNDAVLRISNLADQVGIKAIMVHAIDGKARAFYEYFGFVQSPIAANTLFYKI

Nucleotide

• Length: 483 bp

>T163103 NZ_CP072384:2976716-2977114 [Arachnia rubra]
ATGATCCGCTATCTCCTGGACGCCAACGTCTTGATAGCCCTCACCGCAGCCGAACACGAACACCATGAGGCCGCAGGTGA
GTGGCTCGCAGAAACCACCCAGTTCGCGACCTGCCCCACCACAGAAAGTGCTCTACTGCGTTTCCTCCTTGGTTTGGGCG
AACCAGCCACCATGGCTCTGGAACGCATCCGGCTCATCAGGGCACTCCCTGCTGCCGAGTTCTGGCCCAGCGACCTGTCA
TACGCCGATATCGACGCCACTGGCCTGATCGGTCACCGCCAAGCCACAGATCTCTATCTCGTCTCCCTGGCTGCCGCGCA
CGGCGGACTCCTCGCCACCTTCGACAAGGCCCTTCATGCCTCCCGACCAGACCTGACCTTTCTGATCCCTCGCCCATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11515.11 Da
• Isoelectric Point: 5.8990

>AT163103 WP_168246960.1 NZ_CP056244:2403308-2403604 [Citrobacter freundii]
MEKLMQTSARKPTRDKQINIRATDEERAVIDYAASLVNKNRTDFIIEKAVHEAQNIILDQRVFVLDDARYQSFIEQLEAP
VQNVEGRQRLMDVKPEWK

Nucleotide

• Length: 297 bp

>AT163103 NZ_CP072384:2976486-2976719 [Arachnia rubra]
ATGCGCACCACGGTCGATCTACCCCCTGCAGTCCGAGAAAGAGCACAGGAGATAGCTCAACTACAGGGCCGCTCACTATC
AGCCGTCGTAGCAGAGCTGGCGACCCGGGGGCTTGCCCAGCTCGATGACCACCCAGCCATCATCGAAACCGATCCCCGCA
CCGGCTTCCCCGTTCTACGCATTGGCAGATCCATCACAAACGAACAGGTTGCAGACCTCATCGAGGAGTCATGA