Detailed information of TA system TA163092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 270092..270678
• Replicon: chromosome
• Accession: NZ_CP056244
• Organism: Citrobacter freundii strain RHBSTW-00923

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HV327_RS01285
• Protein ID: WP_117342479.1
• Coordinates: 270310..270678 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HV327_RS01280
• Protein ID: WP_096759232.1
• Coordinates: 270092..270313 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV327_RS01255	265149..266258	+	1110	WP_115257271.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HV327_RS01260	266306..267232	+	927	WP_096759229.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HV327_RS01265	267229..268509	+	1281	WP_096759230.1	branched chain amino acid ABC transporter permease LivM	-
HV327_RS01270	268506..269273	+	768	WP_096759231.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HV327_RS01275	269291..270004	+	714	WP_096759502.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HV327_RS01280	270092..270313	+	222	WP_096759232.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV327_RS01285	270310..270678	+	369	WP_117342479.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV327_RS01290	270932..272248	+	1317	WP_096759233.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HV327_RS01295	272348..273235	+	888	WP_096759234.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HV327_RS01300	273232..274077	+	846	WP_174361255.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HV327_RS01305	274079..275149	+	1071	WP_115257275.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	268506..275889	7383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (24-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13624.90 Da
• Isoelectric Point: 7.4033

>T163092 WP_117342479.1 NZ_CP056244:270310-270678 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIALSANPDFVAMTVEAAAGRLSLEQIAHRLRQ

Nucleotide

• Length: 369 bp

>T163092 NZ_CP072377:c1071308-1070988 [Leptotrichia sp. oral taxon 218]
ATGAGGGATAAAAAAGAAGAGTATAAATATTCTATTATTCTTACTGGGAAATTTAAAAAACATTTAAAAAATCTAAAAAA
ACAGAAAAAAGATTTAAAATTATTGGAAAGTATCATCTCAATATTGGCAAAAGGAGAAAAACTTCCTCCTAAAAACAAGG
ATCATAAATTAGTCAATAATTATGATGGAGCAAGAGAATGTCATATAACTCCAGATTGGTTATTAATCTACGAGATAGTT
GAAGATAAATTAATATTGATAAAACAATTTTATCAAGAAAGGAAAACAAAAAAATGGAAAAGAAAATATACAAAGCAGTA
A

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8260.32 Da
• Isoelectric Point: 4.7769

>AT163092 WP_096759232.1 NZ_CP056244:270092-270313 [Citrobacter freundii]
MRTVNYSEARQNLAEVLDSAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT163092 NZ_CP072377:c1071588-1071298 [Leptotrichia sp. oral taxon 218]
ATGGCAACAGTTAATATAAGTATAAAAATAGATGAAGAAACTAAAAAAGAAGCACAGAAATTATTTAAAGATTTAGGATT
AAGCCTTAGTACAACAATTAATATATTTTTAAAACAGGCTATAAGAGAAAAAGGAATTCCCTTTTACATAAGTTCTCTAC
CTGAAAATTCAGAATTGGTTCAAGCATTTGAAGAAGCTAAACAAATTAAGAAAAATCCTTCAAATTATAAATCGTACAGT
AGTCCAGAAGAAATGTTTAAAGATGTCTTGGGAGAAGATTATGAGGGATAA